牛蒡苷元对猪圆环病毒2型感染仔猪体内病毒复制及组织病理影响

doi:10.11843/j.issn.0366-6964.2018.12.016

Abstract

Abstract:

This experiment was conducted to study the effects of arctigenin (ACT) on viral replication and histopathology after porcine circovirus type 2 (PCV2) infection of piglets. Sixteen 30-day-old healthy piglets were randomly assigned to 4 groups. Drug treated groups were intramuscular injected of ACT on dose of 20 and 50 μg·kg^-1 respectively. Control group and virus challenge group were intramuscular injected with normal saline. After continuous injection for 3 days, the two drug treated groups and virus challenge group were inoculated with PCV2 and control group were inoculated with normal saline. The peripheral blood samples were collected on day -2, 0, 7, 12, 21 and the viral load in those serums were detected by qPCR. The piglets were sacrificed on day 21 after PCV2 infection, and the lung, spleen, tonsilla, mesenterium lymph nodes, inguinal lymph nodes were collected for PCV2 quantitative detection and histopathological observation. The results showed that both ACT treated groups could significantly inhibit the copy number of PCV2 in piglets peripheral blood after 7, 14 and 21 days of infection (P<0.05 or P<0.01). The results of viral load in tissues showed that, PCV2 proliferation in the tissues of inguinal lymph nodes (P<0.05) and mesenteric lymph nodes (P<0.05) were significantly inhibited in the drug group 1 (20 μg·kg^-1), and the viral proliferation in inguinal lymph nodes (P<0.001), mesenteric lymph nodes (P<0.01), tonsils (P<0.05) and lungs (P<0.05) were significantly inhibited in the dose group 2 (50 μg·kg^-1). Further histopathological sections and histopathological scoring results showed that two doses of ACT could reduce the pathological damage of the above-mentioned organ tissue caused by PCV2 infection. These results showed that ACT had a significant inhibitory effect on the proliferation of PCV2 in artificially infected piglets and could reduce the pathological changes caused by PCV2 infection.

CLC Number:

CHEN Jie, WU Li-jun, CHEN Xia-bing, LIU Xiao-li, JIN Er-guang, SHAO Zhi-yong, YANG Wen-hai, HE Bin, TONG Wei-wen, LIU Wu, YANG Han-chun. Effects of Arctigenin on Viral Replication and Histopathology after Porcine Circovirus Type 2 Infection of Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2672-2679.

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Effects of Arctigenin on Viral Replication and Histopathology after Porcine Circovirus Type 2 Infection of Piglets

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